Fig. 8. The expression of fz8a is negatively regulated by Wnt8b. (A) Schematic representation of the transplantation experiment. A small group of 5-10 cells that overexpresses wnt8b was drawn up from a labelled midblastula donor embryo and transplanted into the blastoderm of the midblastula host embryos. (B) The control donor cells cannot affect fz8a expression in the anterior neuroectoderm of the recipient embryo (left), while transplanted cells that overexpress wnt8b strongly suppress fz8a expression in the anterior neuroectoderm of the 100% epiboly stage of host embryos (right). The transplanted embryonic cells are visualised in brown. The expression of fz8a in wild type (WT) (left) and headless (hdl) mutant (right) at the 30% epiboly stage (C) and 100% epiboly stage (D). In hdl mutant embryos, fz8a expression in the neuroectoderm is remarkably decreased.