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Fig. 8. BrdU incorporation is strongly inhibited in salivary glands when {Delta}DMyb, but not DMyb, is ectopically expressed. Salivary glands dissected from larvae between 72 and 96 hours AED were double-stained with DAPI to visualize nuclei (left panels) and for DNA synthesis by BrdU incorporation (right panels). Imaginal ring cells (ir) are shown at higher magnification in inset panels. BrdU incorporation was detected in both imaginal ring and salivary gland (sg) nuclei in (A) control sd-Gal4/+ glands and in (B) sd-Gal4/UAS-DMyb glands. However, BrdU incorporation was only detected in imaginal ring nuclei and not in salivary gland nuclei in (C) sd-Gal4/UAS-{Delta}DMyb and (D) fkh-Gal4/UAS-{Delta}DMyb glands. (E) Heat-shock induction of E2F/DP expression in sd-Gal4/UAS-{Delta}DMyb; HS-E2F, HS-DP/+ glands was able to override the inhibition of DNA synthesis in salivary gland nuclei, but also induced increased levels of BrdU incorporation in fat body (fb) nuclei, leading to excess endoreduplication in these cells. Examples of these fat body nuclei are indicated with arrowheads. Scale bars: in A (left), 0.05 mm for A-E; in A (inset), 0.025 mm for A-E.