Fig. 3. Schiff staining of ‘clearing’ at the blade sheath boundary. (A) Rolled leaf1-PB mutant with region of pale tissue indicated by an arrowhead. (B) Abaxial view of veins at the blade-sheath boundary of a wild-type leaf, as observed with Schiff staining. (C) Abaxial view of blade-sheath boundary in a Rld1 leaf at region of pale tissue (arrowhead in A), located in same longitudinal region as the ligule flap (lf). Schiff-stained vasculature in Rld1-PB leaf shows lack of intermediate and transverse veins (black arrowhead) in this ‘clearing.’ (D) Enlarged view of boxed area in B, showing intermediate veins (iv) and transverse veins (tv) between the lateral veins (lv). (E) Enlarged view of boxed area in C showing lack of intermediate and transverse veins in the region of the ‘clearing’ (black arrowhead).