Fig. 6. Construction and phenotypes of genetic mosaics. (A) Seeds were X-irradiated to generate mosaic plants. (B) Cartoon of a cell showing a pair of chromosome 9 and relative positions of alleles of rld1 and wlu4. The arrow indicates an X-ray breakage event. The somatic loss of the 9L arm will result in a lineage of cells mutant for wlu4 (white) and no longer carrying the mutant Rld1-O allele. (C) Mature leaf from plant heterozygous for Rld1-O and wlu4 (phenotype is Rolled leaf1 and green) with a white sector (hemizygous for rld1⁺wlu4). (D) Schematic showing cross section of a leaf (DV axis) with the five layers (TL1-TL5), and tissue types indicated. (E) Cartoons representing the different classes (i to xi) of leaf sectors found in X-irradiated Rolled leaf1-O plants, white indicates the rld1⁺wlu4 genotype and green indicates the Rld1-OWlu4⁺ genotype. The resulting phenotype (wild-type (wt) or Rolled leaf1(Rld1)) of each of the sectors and the total numbers for each sector class are shown. Sectors within each class came from different chromosome breakage events.