
Fig. 5. Expression of bon/mixer is required for casanova expression. (A,B) Dorsal views, 70-80% epiboly. Compared with the control (A), casanova is normally expressed in most faust mutant embryos, but is sometimes slightly reduced (B). (C,D) Lateral views, 40% epiboly. (E,F) Dorsal views, tail bud. Compared with the control (C,E), there are many fewer cas positive cells in bon mutant embryos from late blastula (D) to late gastrula (F). (G-J) Dorsal views, 70-80% epiboly. Compared with the control (G,I), Xmixer overexpression increases the number of cells expressing casanova in wild-type embryos (H) but not in MZoep mutants (J). (K,L) Combination of Xmixer and fau/gata5 (L, compare with control K) induces, in a few embryos (5%, n=20), a very small number of cas positive cells, which never reach the YSL.