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Fig. 5. PITX2 enhances Gata2 transcription. The fold increase (y-axis) of luciferase gene activity compared to control (transfection of empty vectors only) is shown. Three PITX2 isoform expression vectors were individually cotransfected with Gata2 luciferase construct into {alpha}T3-1 (A) or CV-1 cells (B). PITX2 showed a significant induction of Gata2 luciferase expression in both cell lines (A,B; P<0.05). Different PITX2 isoforms may have different transactivation properties on Gata2 promoter (A). Asterisk indicates statistically significant induction compared to basal level (transfection of reporter construct only without PITX2 expression vector, P<0.05). Results from more than 4 independent experiments were averaged.