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Fig. 1. prd is essential for accessory gland formation. (A,D) Accessory glands (ag) dissected from wild-type (A) and Df(2L)Prl/prd2.45 male rescued by one copy of the prd-SN20 transgene (D). (B,C) Highly underdeveloped (B) or absence (C) of accessory glands in Df(2L)Prl/prd2.45 males rescued to adulthood by two copies of prd-Gsb (B) or prdRes (C) transgenes. (E,F) Complete rescue of accessory glands by one copy of prd-mf5 transgene in Df(2L)Prl prd-mf5/prd2.45 males rescued to adulthood by two copies of prd-Gsb (E) or prdRes (F) transgenes. Testes and seminal vesicles connecting them to the anterior end of the ejaculatory duct (ed) have been removed here and in Fig. 3, Fig. 4, Fig. 5.