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Fig. 1. Dm myb mutants have missing and improperly oriented abdominal bristles, and patches of undifferentiated tissue. (A-H) Comparison of wild-type and mutant abdomens. Dorsal (A-D,H) and ventral (E-G) cuticles from female adult abdomens: control w/Df(1)sd72a (A,E) flies raised at 24°C; myb2 (B,F) flies raised at 24°C; (C) myb1 raised at 18°C; (H) esg mutant (esgk0060/Df(2L)TE35D-2) raised at 24°C; pharate adults of myb2/Df(1)sd72a raised at 24°C (D); and pharate adults of myb2 raised at 28°C (G). (I-L) Comparison of wild-type and mutant abdominal histoblast nuclei in third instar larvae raised at 24°C. All nuclei within the field were visualized by DAPI staining. (I'-L') Abdominal histoblast nest cells were identified by using ß-galactosidase antibodies and the enhancer trap insert esgP3. (I) Control w; esgP3/+; (J) myb2; esgP3/+; (K) myb2/Df(1)sd72a; esgP3/+; (L) esgVS8/esgP3. Scale bars: in A, 0.5 mm for A-H; in I', 0.05 mm in I-L'.