Fig. 6. brat overexpression increases cell size but inhibits clone growth. (A-D) Flow cytometric analysis of dissociated wing imaginal disc cells containing clones overexpressing the indicated genes. In all graphs, the x-axis shows forward light scatter, an indicator of relative cell size, and the y-axis shows cell counts. Overexpression of P35 regularly results in a small decrease in cell size relative to controls that is probably due to a deleterious effect on cell metabolism. (E) Control or UAS-brat clones were induced at 48 hours AED and analyzed 67 hours later. Clone areas were measured using Adobe Photoshop and the average pixel area is plotted with standard error. Eighty-eight control and 77 UAS-brat clones were measured. Genotypes are y w hs-FLP¹²²; Sp/+; Act>CD2>Gal4 UAS-GFP/+ (A), y w hs-FLP¹²²; Act>CD2>Gal4 UAS-GFP/UAS-brat(5) (B), y w hs-FLP¹²²; Sp/UAS-P35; Act>CD2>Gal4 UAS-GFP/+ (C and ‘control’ in E), and y w hs-FLP¹²²; +/UAS-P35; Act>CD2>Gal4 UAS-GFP/UAS-brat(5) (D and ‘UAS-brat’ in E).