Fig. 5. Grafted cells express markers of differentiation correctly. Embryos are doubly stained with X-gal (light blue; donor cells), and antisense riboprobes as indicated (purple). (A,C,E,G) Whole embryo; (B,D,F,H) transverse section of the adjoining embryo at the level shown by the lines in A,C,E,G. Left hand insets in B,D,F,H show high power images of the regions indicated by black arrowheads. Co-expressing cells are indicated by blue arrowheads. (A-D) Embryos resulting from graft of 8.5 d.p.c. node. (A,B) Embryo hybridised with T riboprobe. Like unlabelled host cells, donor cells in the notochord express T, while those in the ventral neural tube (asterisk and upper right-hand corner of inset) do not. (C,D) Embryo hybridised with Shh riboprobe. Donor cells correctly express Shh in the floorplate (compare floorplate in B with that in D). A comparable high magnification image in a control unlabelled embryo is shown (right-hand inset in D). (E-H) Embryos receiving grafts of 10.5 d.p.c. CNH. (E,F) Embryo hybridised with a Shh riboprobe. Donor cells in the notochord express Shh. (G,H) Embryo hybridised with Dll1 riboprobe. Donor cells in dorsal paraxial mesoderm express Dll1, while those outside the region of host cells expressing Dll1 do not (arrow). Asterisks indicate ventral neural tube.