Fig. 7. Expression patterns of transgenes including sequences in the Abd-B m 5' regulatory region. (A) The Abd-B upstream regulatory region and the different genomic fragments used to make the constructs. Coordinates, symbols of restriction sites and transcription start site as in Fig. 1. (B-H) Embryos stained with a anti-ß-galactosidase antibody at the germ band extended (B-D) and germ band retracted (E-G) stages. (B) Embryo transformed with a construct containing the Abd-Bpp fragment and the lacZ gene (Abd-Bpp white) showing the basal expression pattern. (C) Embryos with the BEAbd-BppX2 construct. There is strong expression of ß-galactosidase in PS13 at the germband extended stage (arrow). (D) Embryos transformed with the BEUbxppB4 construct. Note that now there is no strong expression in PS13. After germband retraction, the three constructs show similar strong expression in anterior and posterior spiracles (E-G, arrows).