Fig. 6. dek1-D, crinkly4-1231 double mutant analysis. (A,B) Scanning electron micrographs of a typical cr4-1231 single mutant. Arrows in A denote a bulliform row. The boxed area is enlarged in B. The arrows in B indicate cells with mild bulliform-like surface ridges. Note the aberrant cell shapes and the mismatched crenulations of neighboring cell sidewalls. (C) A dek1-D single mutant. Some cells appear normal, reflecting the leaky allele and mild expression. (D) A double mutant showing primarily the dek1-D phenotype. Most epidermal cells show the surface features of bulliform cells and lack the irregular morphology of cr4. (E) Over-expanded rectangular cells that lack interlocking crenulations along their sidewalls. (F) Highly irregular cells on a double mutant with a strong phenotype. (G) Cross section of a cr4-1231 single mutant. (H) A typical cross section through a double mutant. (I) A cross section through a strong double mutant. The arrow highlights an example of apparent multiple epidermal cell layers. (J) RT-dependent PCR amplification of Cr4 transcripts from wild-type and dek1-792 mutant endosperm. Lane 1, dek1-792 mutant endosperm, with reverse transcriptase (+RT); lane 2, wild-type endosperm (+RT); lane 3, Cr4 cDNA plasmid template; lane 4, dek1-792 RNA (—RT); lane 5, wild-type RNA (—RT). Scale bars 100 µm (A-D,G-I); 10 µm (E,F).