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Fig. 2. Northern blot analysis. (A) Xlim-1/3m (3m) and Xlim-1 plus Ldb1 initiate expression of Xror2 and chordin in animal caps. (B) BMP antagonists, chordin (chd) and noggin (nog), initiate expression of Xror2 in animal caps. (C) Activation of Xror2 expression by Xlim-1 requires protein synthesis. Animal caps (stages 8-8.5) were treated with CHX and 30 minutes later with DEX until the equivalent of stage 11. In A-C, Amounts of injected mRNA (pg/embryo): globin, 250-1000; 3m (H) or 3m, 250; 3m (L), 125; Xlim1+Ldb1(H), 250 each; Xlim1+Ldb1(L), 125 each; chordin, 250; noggin, 125; GR-{Delta}NA, 250. (D) Developmental expression pattern of Xror2. st., developmental stage; whole, whole embryos. Probes are indicated on the right-hand side. Ethidium bromide-stained 18S rRNA is for loading control.