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Fig. 2. Morphology after Lmx1b and Wnt1 misexpression. (A-C) Morphology of Lmx1b-misexpressed embryo at E7.5. Dorsal view (A); view from the caudal side (B); transverse section stained with Hematoxylin-Eosin (C). Expansion of the tectum, torus semicircularis and rhombic lip are seen on the experimental side. (D-H) Morphology after Wnt1 misexpression. E3.5 (48 hours after electroporation; D,E). E14.5 (13 days after electroporation; F-H). Dorsal view (D,F,G); view from the caudal side (E); horizontal section stained with Hematoxylin-Eosin (H). Extra folia (arrows on H) were formed in the cerebellum by Wnt1 misexpression. Scale bars: 2 mm (F), 1 mm (B,G), 500 µm (C,E,H). cer, cerebellum; cont., control side; exp., experimental side; met, metencephalon; rl, rhombic lip; tec, tectum; ts, torus semicircularis.