Fig. 8. Effects of Otx2, Fgf8 and Gbx2 on Lmx1b expression. (A-C) In situ hybridization for Lmx1b (blue) and Otx2 (red) on the same embryo at 24 hours after electroporation of pMiw-Otx2. Lmx1b is induced by Otx2 misexpression in the metencephalon (B). (D-F) In situ hybridization for Lmx1b (blue) and Fgf8 (red) on the same embryo at 24 hours after electroporation of pMiw-Fgf8b. Lmx1b is induced by Fgf8b misexpression in the diencephalon and mesencephalon broadly (E). (G,H) In situ hybridization for Lmx1b (blue) on the embryo at 36 hours after electroporation of pMiw-Fgf8b. (H) Lmx1b expression appears as a half ring in the diencephalon. In addition, endogenous Lmx1b expression in the isthmus is also disappeared (H). (I-K) In situ hybridization for Lmx1b (blue) and Gbx2 (red) on the same embryo at 24 hours after electroporation of pMiw-Gbx2. (K) High-power magnification of J. Lmx1b was repressed by Gbx2 misexpression (indicated by arrows on K). (A,D,G,I) Views from the control side. (B,C,E,F,H,J,K) Views from the experimental sides. Scale bars: 500 µm in F,H; 250 µm in C,J; 100 µm in K.