Fig. 5. Expression of Hoxd11 and Hoxd10 in Hoxd^{RXII-del(13-12)} animals. (A,B) Lateral and dorsal views, respectively, of an 11.5 dpc foetus analysed for Evx2 transcripts. A strong expression is detected in the developing digits, in a domain that is identical to the distal expression domain of Hoxd11 (D). Evx2 transcripts are also observed in columns of cells with the developing spinal cord, up to the posterior hindbrain (black arrowhead), as well as in a region encompassing the cerebellar anlage (black arrow) up to the isthmus. More rostrally, transcripts are found in the mesencephalon, (white arrow) (Dollé et al., 1994). (C) Control embryo of the same age hybridised with a probe specific for Hoxd11 RNA. None of this CNS domain is observed. (D-F) Ectopic expression of Hoxd11 (D,E) and Hoxd10 (F) in the CNS. In addition to the expected expression patterns in limbs and developing trunk (D), these latter genes show clear ectopic activation in domains virtually identical to those where Evx2 is expressed (D-F, compare with A and B), indicating that they now are under the control of Evx2 neural enhancers.