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Fig. 2. Meiotic germ cells interfere with cord formation. Germ cells and somatic cells were isolated using an immunomagnetic cell sorting system. Somatic cell fractions from 12.5 dpc XY gonads were aggregated with 11.5 dpc XX germ cell fraction (A), 11.5 dpc XY germ cell fraction (B), 14.5 dpc XX somatic fraction (C) or 14.5 dpc XX germ cell fraction (D) and cultured for 48 hours. Formation of testis cords was shown by immunostaining for laminin (green), a component of the basal lamina of testis cords (red arrows).