Fig. 5. GT hypoplasia of Nog mutant mice. (A,B) Nog mutant mice showed GT hypoplasia at 16.5 dpc. (C-F) A reduction of Wnt5a and Fgf8 expression in the developing external genitalia region of Nog mutants compared with wild type at 12.5 dpc (for Wnt5a) and at 12.0 dpc (for Fgf8). (G) Reduced cell proliferation of Nog mutant mice GT. The number of phospho-histone H3 immunopositive cells decreased to 60% compared with wild type. (H,I) TUNEL analysis was performed on Nog mutant at 12.5 dpc. Apoptotic-cells were not detected in distal mesenchyme of Nog mutants. (A,B) Lateral views; (C-F) ventral views; (H,I) coronal sections. Scale bars: 650 µm in A,B; 250 µm in C-F; 200 µm in H,I.