Fig. 2. EPg J3-220 and EPg 35635 target the dan and danr genes, which encode related pipsqueak-motif-containing proteins. (A) (Top) the genomic region containing the dan and danr genes. The insertion points of EPg J3-220 and EPg 35635 (green triangles) lie 45 kb apart. Predicted genes in the region are shown in blue. (Middle) Red arrowheads indicate the positions of the primer pairs used for genomic PCR to determine the breakpoints of excision mutations; broken red lines between each primer pair indicate the predicted wild-type sizes of the genomic PCR fragments. (Bottom) Boxes show the extent of the genomic regions deleted in the dan danr^ex56 and danr^ex35 alleles. (B) Alignment of the Dan and Danr sequences showing regions of similarity. Note that the two proteins are most similar at the N terminus. The amino acid change in dan^ems3 is indicated in red above the alignment. (C) Alignment of pipsqueak motifs from Dan, Danr, Drosophila Pipsqueak, and the transposases Drosophila Pogo and human CENP-B. Asterisks below the alignment indicate identical residues; colons and periods indicate conserved residues (ClustalW). The arrow indicates the residue mutated in dan^ems3.