Fig. 6. Flamingo overexpression results in axon retraction. (A) Adult mushroom body axon lobes as visualized by pan-MB expression of mCD8-GFP (green) using GAL4-OK107; double labeled with FasII (in red) (B) Overexpression of Fmi using GAL4-OK107 results in the loss of the dorsal lobe (horizontal arrow). (C-F) Representative images of mushroom bodies overexpressing Fmi at different developmental stages as indicated. APF, after puparium formation. (G) Quantification of dorsal lobe phenotypes. n=50, 8, 16, 12, 50 for the five stages quantified. A is adapted from Billuart et al. (Billuart et al., 2001). Copyright (2001), with permission from Elsevier Science.