Fig. 6. Repression of LMC neuron specification by Lim3 but not by MNR2 or HB9. (A-C) Electroporation of MNR2 within the normal domain of LMC neuron generation at brachial levels of the spinal cord does not maintain Lim3 expression in postmitotic LMC neurons. B and C, enlarged view of boxed area in A. (D) Electroporation of HB9 within the normal domain of LMC neuron generation at brachial levels of the spinal cord does not maintain Lim3 expression in postmitotic LMC neurons. (E-H) Electroporation of MNR2 within the normal domain of LMC neuron generation at brachial levels of the spinal cord does not repress expression of RALDH2 or the generation of motor neurons that co-express Isl2 and Lim1. G and H, enlarged view of boxed area in E. (I-L) Electroporation of Lim3 within the normal domain of LMC neuron generation at brachial levels of the spinal cord represses expression of RALDH2 and the generation of motor neurons that co-express Isl2 and Lim1. K and L, enlarged view of boxed area in J. Images are representative of 10-30 electroporated embryos.