Fig. 6. Differential regulation of Wg and Vg in wing and haltere discs. (A,B) vg-GAL4/UAS-DN-TCF/pan-wing (A) and -haltere (B) discs stained with anti-Wg antibodies. Misexpression of DN-TCF/pan downregulates Wg expression at the wing disc DV boundary, but not in haltere discs. (C,D) vg¹/vg¹ haltere discs stained for Vg (C) and Wg (D). Note the absence of Wg expression at the DV boundary, which suggests that Wg expression in haltere discs is dependent on Vg. (E) vg-GAL4/UAS-DN-TCF/pan-haltere disc stained for Vg. DN-TCF/pan does not have any affect on Vg expression, which suggests that its expression at the haltere DV boundary, unlike in wing discs, is independent of Wg signaling.