Fig. 3. Late gestation ß1D1D ki/ki embryos and newborn pups are thinner/shorter than their littermates and exhibit a reduction in muscle mass. (A,B) Gross morphology of E18.5 embryos (A) and newborns (B). Note the curved posture of the E18.5 ß1D1D ki/ki embryo. (C-L) Transverse Haematoxylin and Eosin-stained sections of E18.5 wild-type (C,E,G,I,K) and ß1D1D ki/ki (D,F,H,J,L) embryos showing lung (C,D), heart (E,F), thoraxic body wall (G,H), shoulder (I,J) and diaphragm (K,L) muscles. Dg, diaphragm; Dl, deltoideus; e, esophagus; I, intercostal; lg, lung; P, pectoral; r, rib; s, scapula. Scale bar: 200 µm.