Fig. 4. Effects of pharmacological reagents on cystic bulge movement. Speed before treatment is the average speed of cystic bulge movement for 40 minutes before treatment. Speed during treatment is the average speed from 40 minutes to 80 minutes after application of drug in the same cyst. In each column, average speeds of 5-10 cysts are shown. (A) Effects of drugs that alter actin dynamics. Both LTA and jasplakinolide (JSP) inhibited cystic bulge movement in a dose-dependent manner. LTA (6 µM) completely stopped movement 20 minutes after application. (B) Effects of drugs that alter microtubule dynamics. Nocodazole (noc), colchicine (col) and taxol show minimal effect on cystic bulge movement. (C) Effect of inhibitors of motor proteins. Kinesin inhibitor monastrol and myosin inhibitor BDM did not inhibit movement of the cystic bulge.