Fig. 3. Tethered lacI-HP1 causes ectopic associations. Third instar larvae of stocks 157.1, 157.4.112 and P2.5 expressing lacI-HP1 were stained with antibodies to lacI (green). Arrows indicate the locations of lac repeats. The percent of nuclei showing ectopic associations, out of 60 nuclei scored for each cytological position, are shown at the bottom of each panel. Associations were observed between the tethered site and other HP1-containing sites (A,B), but were also observed at sites not enriched with HP1 (C).