Fig. 4. Alterations of Her6 and Her4 expression and/or activity disrupt the coordinate cyclic expression of deltaC and her1. Flat-mounted embryos at 10-12 ss. Two-cell stage embryos were injected in one cell only. In all cases, the injected side is on the right. Red (A,C,D,F,G) or brown (H,J,K) staining labels the injected side. The pattern of sharp deltaC stripes is disrupted in the anterior PSM of embryos injected with wt-her6 (A), her6-VP16 (D,E) or wt-her4 (H,I). In the anterior PSM of her6-VP16- (E') and wt-her4- (I') injected embryos, a random mixture of cells expressing deltaC at different levels is found. (E',I') Higher magnifications of the boxed regions in E and I, respectively. The sharp stripes of her1 expression are replaced by a random mixture of her1-positive and her1-negative cells throughout the PSM of embryos injected with wt-her6 (B-C''), her6-VP16 (F-G'') or wt-her4 (J-K''). (C',C'',G',G'',K',K'') Magnified views of the boxed areas in C, G and K, respectively, to allow a better comparison between injected and noninjected side of the embryos.