Fig. 2. slmb loss of function provokes a wide array of ovarian phenotypes. (A) DAPI staining of a wild-type ovariole (interfollicular stalks are marked by arrows). (B) Some egg chambers from females with slmb germline clones exhibit premature apoptosis (arrow) indicated by the occurrence of fragmented nurse cell nuclei (C, arrowhead). (D) Wild-type follicles always contain 15 nurse cells and one oocyte; in slmb germline clones, egg chambers with decreased (E) or increased (F) number of germ cells are frequently observed, whereas in other follicles germ cell nuclei were heterogeneous in size (G). (H) In females bearing FC clones, egg chambers with two oocytes occur (arrows). (I) Wild-type germarium in which FC are stained for Fas3; the cyst in region IIb shows the typical lens shape (white asterisk) and once in region III the cyst re-shapes into a sphere (yellow asterisk). (J) In slmb somatic clones, the general shape of the germarium is frequently altered and cysts are placed side by side in regions II and III (asterisks). slmb FC clones exhibit a wide array of defects, including abnormal morphology of egg chambers (K; compare with D), oocytes that are mispositioned (L,M, arrows) and lack of interfollicular stalks between adjacent follicles (N,O, arrows; compare with A). (P) slmb/CSN5 genetic interactions; slmb² germline clones that are heterozygous for CSN5^L4032 display enhanced phenotypes such as enlarged germaria (arrow).