Fig. 7. Broad-Complex (BR-C) expression and ectopic activation of the Dpp pathway. (A) mRNA in situ hybridization in a stage 10 wild-type egg chamber showing that BR-C is expressed in two patches of dorsoanterior FC. (B-D) In follicles bearing slmb somatic clones, BR-C expression is in some cases expanded (B, arrow) and in others reduced (C, arrow) or split (D, arrows). (E,F) Expression of the dad-lacZ enhancer trap in wild-type ovarioles. (E) In the follicular epithelium, expression is first observed at stage 8 in anterior FC (arrow), being undetectable at stage 6. (F) At stage 9, dad-lacZ can be seen in migrating anterior FC (black arrow) and at stage 10 in stretched cells (arrowhead) and in centripetal cells localized at the anterior border of the oocyte (white arrow). (G) In ovarioles bearing slmb mutant clones, expression of dad-lacZ occurs prematurely at stage 6 (arrow); at stage 10 (H), expression of the enhancer trap often expands posteriorly (arrow). (I) Positive GFP labeling of a slmb mutant clone showing that GFP signal overlaps with ectopic expression of dad-lacZ in the clone. (J,K) In wild-type ovaries, expression of Medea occurs at stage 9 in anterior FC that migrate towards the oocyte (J, arrow) and at stage 10 (K) in all FC, being much stronger in stretched cells (arrowhead) and in centripetal cells localized at the anterior border of the oocyte (arrow). (L) In slmb mutant follicles, strong Medea signal was detected in ectopic patches of columnar FC, at stage 10 (arrow). (M) Positive GFP labeling of the slmb mutant clone overlaps with this patch.