Fig. 2. hGfap-cre^+/–;Pten^loxp/loxp mice show severe granule neuron ectopia but normal neuronal differentiation. (A) Sagittal section of P14 mouse cerebella (rostral is upwards and anterior is towards the left). High-magnification view of Pten mutant cerebella (from framed areas in A, a,b) showed increased number of cells in the ML (A,b') compared with littermate controls (A,a'). (B) p27 immunostaining confirmed ectopic cells in Pten mutants were granule neurons. (C) The expression patterns for axon extension (Tuj1) and early granule neuron differentiation (TAG1). Control versus Pten mutant. (D) The expression of GABA_A receptor 6 at different time points. Control versus Pten mutant. EGL, external granule layer; ML, molecular layer; IGL, internal granule layer. Scale bar: 50 µm.