Fig. 6. Vessel morphology in mouse tissues treated with VEGF-Trap. Mouse pups were treated every 2 days for 7 days with 25 mg/kg ip of VEGF-Trap protein. Tissue is from P14 mice, with blood vessels immunostained for PECAM (green) and smooth muscle cell actin (red/orange). (A,B) Whole-mount views of tracheas, showing straight vessels (arrowheads) across the cartilaginous rings in control mice (A). The vessels across the cartilaginous region (asterisks) in tracheas of VEGF-Trap-treated mice (B) are completely absent. (C,D) Cross-sections of tongue, with the upper epidermal surface of the tongue at the upper part of the image. The vessel loops in the dermal papillae (C, arrowheads) in tongues of VEGF-Trap-treated mice are almost abolished (D, asterisks), and the vascularity of the dermis and muscle layers is also reduced.