Fig. 3. Foxa1-deficient prostate has altered epithelial cell population and stromal pattern. (A) Ck5 staining on 4-week-old rescued Foxa1^–/– VP. (B) Ck5-positive basal epithelial cells (arrow) expanded within the null epithelial cords. (C) Ck5 staining on rescued wild-type VP. (D,E) Dual-staining of p63 (green) and Ck8 (red) on 4-week-old rescued Foxa1^–/– and Foxa1^+/+ DLPs. (F,G) Some Foxa1^–/– cells co-expressed of both markers (arrowhead in F), while control cells expressed these markers separately (G). (H-J) SMA staining on 4-week-old rescued VPs. Expansion in smooth muscle layer (arrowheads) was seen in null prostate (H,I), but not in control (J).