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Fig. 8. Effect of antisense oligonucleotides and anti-pancytokeratin C11 antibody injection on germ plasm and germinal granules in two-cell embryos. (A) Whole-mount in situ hybridization with digoxigenin-labeled antisense Xcat2 RNA probe showing the Xcat2 RNA present in the germinal granules within the germ plasm (arrows). Germ plasm of control (1), antisense Xlsirts ODNs (2), antisense VegT ODN (3) and anti-pancytokeratin C11 antibody (4) -injected embryos. (B) Higher magnification of germ plasm regions showing Xcat2 labeled germinal granules in control embryos (1, arrow); unlabelled or slightly labeled granules in antisense Xlsirts embryos (2, arrows); and labeled large stringy aggregates of germinal granules in antisense VegT (3, arrows) and anti-pancytokeratin C11 antibody (4, arrows) embryos. Scale bars: 340 µm in A; 25 µm in B.