Fig. 2. The pipe^C14 allele leads to the loss of pipe RNA and protein expression in the ovary and the embryo. (A-C) In situ hybridization to a wild-type embryo (A) and to embryos homozygous for pipe^C14 (B) and pipe² (C). A pipe-ST2-specific probe was used. (D-F) An antibody directed against a peptide sequence present in the N terminus of all Pipe isoforms was used for immunostaining of wild-type (D), pipe^C14 homozygous (E) and pipe² homozygous (F) embryos. (G-I) In situ hybridization to stage 10 egg chambers from wild-type (G), pipe^C14/Df(3L)pipe^A13 (H) and pipe²/Df(3L)pipe^A13 (I) females using pipe-ST2-specific sequences as a probe. (J) pipe^C14/Df(3L)pipe^A13 flies exhibit a growth defect relative to their heterozygous siblings. Similarly, wind^T6/wind^RP flies (K) are smaller than their wind/+ siblings. Both results suggest that zygotic expression of Pipe isoforms is required for normal growth.