Fig. 4. Actin and cell morphology at compartment boundaries. Wild-type mid-third instar wing discs, stained with phalloidin to reveal F-actin (green). To enable visualization across the curved surface of the disc in a single panel, the stains shown are projections through different focal planes. White arrows indicate the DV boundary. (A) Disc with elevated apical F-actin staining at the DV boundary clearly visible. A nuclear marker for dorsal cells (ap-lacZ, red) is not visible in apical focal planes, but is visible in more basal focal planes (A'). F-actin staining, however, is neither elevated nor smooth in basal-lateral sections (A',A''). (B-E) Close up of a portion of the DV border, stained for F-actin and E-cadherin (B), F-actin and ß-catenin (C), F-actin and anti-phospho-tyrosine (D), and Enabled and E-cadherin (E). (F) Vertical section through a disc stained for F-actin (green), E-cadherin (red) and ap-lacZ (blue). Arrow indicates F-actin at the DV boundary, which overlaps with E-cadherin.