Fig. 4. Kidneys of Fgf8 hypomorphs are smaller than normal, but contain
nephrons. (A) Comparison of normal, Fgf8neo/neo (mild
hypomorph) and Fgf8neo/null (severe hypomorph) kidneys at
E18.5. (B,C) In situ hybridization assay for Wnt4 RNA in vibratome
sections of E15.5 kidneys. (D) Immunofluorescence assays at E15.5 for
PECAM-positive (red) vasculature and WT1-positive (green) podocyte progenitors
in vibratome sections. (D') A higher magnification view of a renal
corpuscle in D.