Fig. 3. Fgf8 expression in control and T-Cre; Fgf8^Flox/2,3 kidneys. (A) Urogenital tissues of control and mutant female neonates showing abnormally small kidneys. (B) Whole-mount ISH of Fgf8 in a control 11.5 kidney (left, lateral view; right, dorsal view), demonstrating expression adjacent to the ureteric bud. The ureteric bud and kidney are outlined in white and black dashed lines, respectively. (C) Whole-mount ISH using a Fgf8 probe specific for the Cre-deleted sequences (see Fig. 2B) of the E12.0 urogenital region (ventral view) in control (left) and mutant (right). Brackets indicate kidneys. (D) ISH performed on sectioned E14.5 control kidney. (E) Enlargement of the box in D illustrates Fgf8 expression in renal vesicles and newly formed tubules. (F) Whole-mount ISH using a Fgf8 probe specific for Cre-deleted sequences (see Fig. 2B), demonstrating expression in control (left) and mutant (right) E14.5 kidneys. (G) Whole-mount ISH using an Fgf8 probe including sequences outside of the Cre-deleted region (see Fig. 2B), demonstrating continued Fgf8 expression of Fgf8 in both control (left) and mutant (right) E14.5 kidneys. ad, adrenal gland; bl, bladder; cb, comma-shaped body; gl, glomerulus; ki, kidney; mm, metanephric mesenchyme; ov, ovary; rv, renal vesicle; ub, ureteric bud; ur, ureter; ut, uterus.