Fig. 9. Two different Minute mutations affect Atonal expression, whereas
Egfr alleles do not. Eye discs stained for Atonal are shown
inA-C,E,F,H,I,K,L,N,O,Q,R. Clones are negatively marked by ß-gal (white
in D,G,J,M,P, blue in F,I,L,O,R) and are outlined. (A-C) Egfr alleles
in trans to wild type; note normal Atonal expression. (D-R)
hs:Flp-induced clones induced in trans to Minute
chromosomes. (D-O) ß-gal-positive territories are heterozygous for the
Egfr allele and for the Minute mutation. Black (ß-gal
negative) territories are homozygous for the Egfr allele and are wild
type for the Minute mutation. (P-R) ß-gal-positive territories
are heterozygous for the Minute mutation. Black (ß-gal negative)
territories are genetically (but not phenotypically) wild type. No
Minute homozygous cells survive. Mutations are
indicated on the left. Note, the Atonal pattern is not wild type in either the
Egfr homozygous (black in D-O) or the Egfr heterozygous
territories (compare the ß-gal-positive regions to the heterozygous
examples shown in A-C). Also, the Atonal pattern is not wild type in either
the wild-type territories (white arrows in P-R) or the adjacent
Minute heterozygous cells (black arrows in P-R). Note that
Minute(2)53 and Minute(2)56i have different, but
self-consistent dominant effects on Atonal expression. Atonal expression in
the Egfrtsla Minute+ clones is
indistinguishable from that in the two Egfr null alleles. Scale bar:
10 µm.