Fig. 6. Reductions in somatostatin and parvalbumin-expressing interneuron subgroups in NsCre:Shh^Fl/Fl cortex. Shown are examples of immunofluorescence labeling with antibodies to the neuronal marker NeuN (A,A'), a marker of projection neurons, Tbr1 (B,B'), the interneuron marker GABA (C,C'), and three markers of MGE-derived, Nkx2.1-dependent interneuron subgroups, parvalbumin (Pv; D,D'), somatostatin (Som; E,E') and neuropeptide Y (Npy; F,F'). (A-F) Wild type; (A'-F') mutant. Data in G-I was collected from cryosections through the somatosensory cortex of three mutants and three controls at P12. Despite the trend towards a slightly increased density in both NeuN-labeled cells and in all cells in the mutants (G,H), there is a significant reduction in the percentage of neurons that express GABA (H). In the superficial cortex, this reduction is offset by an increased percentage of neurons that express Tbr1. Consistent with the reduction in GABA, reductions in cells expressing Pv, Som and Npy were observed, although after adjustment for multiple comparisons this effect was significant for only Pv and Som in layers 2-4. *P=0.05; **P<0.01. Scale bar in F: 100 µm for A-F.