Fig. 5. Expression of dsc-1(+) in two enteric muscles in sufficient to rescue the defecation defects of dsc-1 mutants. (A) Schematic of construct used to express dsc-1 specifically in the AD and IM. (B) Expression of the construct depicted in (A) in dsc-1(qm133) animals. The dotted line represents the anus. (C) Comparison of the degree of rescue of dsc-1(qm133) mutants obtained by expression of the dsc-1 cDNA specifically in the IM and AD under the NdE-box promoter in comparison to the expression of the dsc-1 genomic region under its own promoter, which expresses dsc-1 in sensory neurons as well as in the two enteric muscles (shown in Fig. 3). Dark gray bars represent transgenic worms, and light gray bars represent the non-transgenic siblings from the transgenic lines. For the left panel, the bars represent the average number of expulsions per six pBocs observed for three independent transgenic lines, and the error bars represent the standard deviations of the means obtained for each transgenic line. For the right panel, the bars represent that average defecation cycle length observed for three independent transgenic lines, and the error bars represent the standard deviations of the means obtained for each transgenic line. Twelve transgenic and 12 non-transgenic animals were scored per line. Scale bar: 10 µm.