Fig. 6. The Exp defect of dsc-1 is due to the lack of exp-1 expression. (A,B) Expression of an exp-1::gfp transcriptional reporter clone (pAB04) (see Beg and Jorgensen, 2003) in wild-type and dsc-1 mutant backgrounds. (The same extra-chromosomal array was expressed in all three backgrounds; the same results were obtained with >4 independent extra-chromosomal arrays.) (C,D) Expression of an integrated unc-49B::gfp translational reporter clone in wild-type and dsc-1 mutant backgrounds. The same integrated array (oxIs22) (see Bamber et al., 1999) was expressed in all three backgrounds. Scale bar: 10 µm. NC, nerve chord; Sph, sphincter. (E) Expression of an Nde-box::exp-1 fusion in exp-1(sa6) and dsc-1(qm133) backgrounds. For the left panel, the bars represent the average number of expulsions per six pBocs observed for three independent transgenic lines, and the error bars represent the standard deviations of the means obtained for each transgenic line. For the right panel, the bars represent that average defecation cycle length observed for three independent transgenic lines, and the error bars represent the standard deviations of the means obtained for each transgenic line. Twelve transgenic and 10 non-transgenic animals were scored per line.