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Fig. 3. ATBF1 induces cell cycle arrest in Neuro 2A cells without inducing apoptosis. (A1-5) HA-tag (red) and BrdU (green) overlap (yellow). (B1-5) BrdU (green) does not overlap with HA-tagged ATBF1 (red) expressions. The HA-tagged ATBF1 positive cells appear with neurite like projections. (C1-5) TUNEL staining (green) after transfection with an HA-tag (red) mock expression vector. (D1-5) TUNEL-positive cell (green) is small compared with HA-tagged ATBF1-positive cells that appear with larger cell bodies and neurite-like projections. (E1-4) FACS analysis after transfection with HA-tag or HA-tagged ATBF1 expression vector. The cells expressing HA-tag or HA-tagged ATBF1 were selected by gating the higher intensity of the green signal (gate: G1 indicated in E1 and E2). PI patterns of selected cells expressing HA-tag (E3) and HA-tagged ATBF1 (E4).