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Fig. 4. PI patterns of P19 cells by FACS analysis corresponding to undifferentiated P19 cells (A), P19 cells after RA treatment for 24 hours in floating culture (B) and differentiated P19 cells on an adhesive dish after 7 days (C). Immunoreactivities of P19 cells for ATBF1 (red) and ß-tubulin (green), overlaid with DAPI staining (blue). Undifferentiated P19 cells (A1). P19 cells after RA treatment for 24 hours in floating culture (B1). Differentiated P19 cells on an adhesive dish after 7 days (C1). ATBF1 (red) and ß-tubulin (green) observed by confocal microscopy. P19 cells express ATBF1 in the cytoplasm after RA treatment for 24 hours in floating culture (B2-4). Differentiated P19 cells express ATBF1 in the nucleus on an adhesive dish after 7 days treatment with RA (C2-4).