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Fig. 4. Regulation of hth expression by eyg. (A) Mutant eyg disc stained with an anti-Hth antibody showing generalised hth expression. (B,C) A mutant eyg- clone (arrows, labelled by the loss of GFP) in the inter-subdomains zone, exhibiting ectopic hth expression. (D-F) Notum region with many clones of eyg-expressing cells, marked with GFP. The clones in the {alpha} subdomain do not affect hth expression, in contrast to those in the ß subdomain where hth is lost. (G-I) Disc of genotype 248-Gal4>UAS-eyg doubly labelled with anti-Eyg (green) and anti-Hth (red), showing loss of hth in the ß but not in the {alpha} subdomain.