Fig. 6. (A) Adult thorax of genotype 248-Gal4>UAS-hth. Although the scutellum remains approximately normal, most of the scutum is lacking. Many microchaetes are missing. (B) Thorax of a 248-Gal4>UAS-eyg fly. As described previously (Aldaz et al., 2003), the scutellum is replaced by a scutum-like pattern in mirror-image orientation. Macrochaetes are present in the duplicated structure. (C) 248-Gal4>UAS-hth UAS-eyg thorax. The only macrochaetes that remain are those in the lateral region (where the Gal4 line is not expressed), while the central region only differentiates microchaetes. (D) Pull-down assay. All three lanes are tested with the Eyg antibody for the presence of Eyg product (80 kDa band). The first lane corresponds to crude extracts. The second lane to the extract incubated with His-agarose, and in the third lane the extract was incubated with His-Hth protein. Eyg is detected in the larval extract and in a complex formed with Hth.