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Fig. 4. Cloacal muscle precursors reside in the hindlimb. (A) Quail leg bud transplanted onto chick hindlimb stump at HH23 results in cloacal muscles consisting of quail cells at HH36. Proximal skeletal elements are of only chick origin (asterisk). (B) Injection of retrovirus into the proximoventral region of the leg bud at stage HH25 gives rise to cloacal muscles (TCL and SCS) labelled with lacZ. There is concomitant signal in the leg muscles. GC, gastrocnemius muscle; LC, levator cloacae; PCE, m. pubocaudalis externus; PCI, m. pubocaudalis internus; SCC, m. sphincter circular; SCS, m. sphincter sling-shaped; TCL, m. transversus cloacae.