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Fig. 6. Experimental evidence for cloacal muscle dependency on leg muscle development. (A) Whole-mount in situ hybridisation with MyoD probe on chick embryo at stage HH34 after surgical leg bud ablation at HH18. Ventral view of the cloacal region shows the unilateral absence of all cloacal muscles on the operated side (arrow). (B) Left operated side, lateral view; (C) right control side, lateral view (mirror image). Unilateral absence of all cloacal muscles on the operated side (asterisk in B) and normal pubocaudal and tail muscles are shown. Orientation is marked: V, ventral; D, dorsal. PCE, m. pubocaudalis externus; PCI, m. pubocaudalis internus.