Fig. 2. chk2 is translationally repressed by orb and vls during oogenesis. The indicated ovary extracts were probed with -Chk2 (Masrouha et al., 2003) and -eIF4A antibodies as a loading control. Protein levels were quantified using a phosphorimager. The upper band in OreR and chk2^null mutants is crossreacting material of unknown identity. Chk2 levels were normalized to eIF4A levels and expressed as a percentage of normalized Chk2 levels in wild type (OreR), which is arbitrarily set at 100%.