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Fig. 6. Scx expression is upregulated in the dorsolateral sclerotome of Sox5-/-; Sox6-/- embryos. Section in situ hybridization for Scx (A,D), Sox9 (B,E), lacZ (C,F) or Pea3 (G,J) on frontal sections through thoracic somites of Sox5+/-; Sox6+/- (A-C,G) or Sox5-/-; Sox6-/- (D-F,J) embryos. Red arrow in D indicates upregulation and expansion of Scx expression. (H,K) Tunnel assays at E10.5 on frontal sections of Sox5+/-; Sox6+/- (H) or Sox5-/-; Sox6-/- (K) embryos. (I,L) Detection of phosphorylated ERK/MAPK on frontal sections at E10.5 on Sox5+/-; Sox6+/- (I) or Sox5-/-; Sox6-/- (L) embryos. Yellow arrows indicate anterior and posterior sclerotome; asterisks indicate dorsal root ganglia. My, myotome. Scale bars: 50 µm.