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Fig. 7. Scx is expressed throughout the undifferentiated Sox5-/-; Sox6-/- skeletal elements. Section in situ hybridization for Scx (A,D,G,J,M,P,O,R), lacZ (C,F,I,L), and Sox9 (N,Q) on sections through E14.5 Sox5+/-; Sox6+/- (A-C,G-I,M-O) or Sox5-/-; Sox6-/- (D-F,J-L,P-R) embryos. (B,E,H,K) Alcian Blue staining. (O,R) Combined section in situ hybridization and immunohistochemistry for Scx (purple) and myosin heavy chain (brown), respectively. (A-F) Transverse sections through vertebrae. Green arrows indicate vertebral bodies; red arrows, neural arches. (G-R) Transverse sections through ribs.