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Fig. 1. Tbx5 and Tbx20 morpholinos block translation of their respective target proteins. (A) TBX5MO and TBX20MO positions relative to Tbx5 and Tbx20 cDNA. (B) Inhibition of TBX5-V5 translation in vitro by TBX5MO. TBX20MO and ControlMO serve as controls. Each reaction contains 1 µg of Tbx5-V5 circular plasmid along with the indicated amounts of MO. (C) Inhibition of TBX20-V5 translation in vitro by TBX20MO. TBX5MO and ControlMO serve as controls. Each reaction contains 1 µg of Tbx20-V5 circular plasmid along with the indicated amounts of MO. (D) Inhibition of TBX5-V5 translation by TBX5MO in animal caps. TBX20MO and ControlMO serve as controls. Probed with anti-V5 and re-probed with anti-PTP1D/SHP2 as a loading control. Embryos injected with 2 ng mRNA and the indicated amounts of MO. (E) Inhibition of TBX20-V5 translation by TBX20MO in animal caps. TBX20MO and ControlMO serve as controls. Probed with anti-V5 and re-probed with anti-PTP1D/SHP2 as a loading control. Embryos injected with 2 ng mRNA and the indicated amounts of MO.